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[KD]G3BP Rabbit mAb

Western blot analysis of G3BP in HeLa(WT) and G3BP-Knockdown HeLa(KD) using G3BP antibody.
Western blot analysis of G3BP in K562, C6, 3T3, Hela lysates using G3BP antibody.
Immunohistochemistry analysis of paraffin-embedded Human colon cancer using G3BP antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Immunocytochemistry analysis of G3BP (green) in Hela using G3BP antibody,and DAPI(blue)

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, FC, IP
Primary Accession	Q13283
Reactivity	Rat, Human, Mouse
Host	Rabbit
Clonality	Monoclonal Antibody
Isotype	IgG
Conjugate	Unconjugated
Purification	Affinity Purified
Calculated MW	52164 Da

Additional Information
Gene ID	10146
Other Names	G3BP1
Dilution	WB~~1:1000-1:5000 IHC-P~~1:50~200 IHC-F~~N/A FC~~1:20-1:50 IP~~1:20
Format	Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	G3BP1 {ECO:0000303\|PubMed:23279204, ECO:0000312\|HGNC:HGNC:30292}
Function	Protein involved in various processes, such as stress granule formation and innate immunity (PubMed:37672657, PubMed:20180778, PubMed:23279204, PubMed:30510222, PubMed:30804210). Plays an essential role in stress granule formation (PubMed:37672657, PubMed:20180778, PubMed:23279204, PubMed:32302570, PubMed:32302571, PubMed:32302572, PubMed:34739333, PubMed:35977029, PubMed:36183834, PubMed:36279435, PubMed:36692217, PubMed:37379838). Stress granules are membraneless compartments that store mRNAs and proteins, such as stalled translation pre-initiation complexes, in response to stress (PubMed:37672657, PubMed:20180778, PubMed:23279204, PubMed:27022092, PubMed:32302570, PubMed:32302571, PubMed:32302572, PubMed:36279435, PubMed:37379838). Promotes formation of stress granules phase-separated membraneless compartment by undergoing liquid-liquid phase separation (LLPS) upon unfolded RNA-binding: functions as a molecular switch that triggers RNA-dependent LLPS in response to a rise in intracellular free RNA concentrations (PubMed:32302570, PubMed:32302571, PubMed:32302572, PubMed:34739333, PubMed:36279435, PubMed:36692217). Also acts as an ATP- and magnesium-dependent helicase: unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency (PubMed:9889278). Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA (PubMed:9889278). Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends (PubMed:9889278). Plays an essential role in innate immunity by promoting CGAS and RIGI activity (PubMed:30510222, PubMed:30804210). Participates in the DNA-triggered cGAS/STING pathway by promoting the DNA binding and activation of CGAS (PubMed:30510222). Triggers the condensation of cGAS, a process probably linked to the formation of membrane-less organelles (PubMed:34779554). Also enhances RIGI-induced type I interferon production probably by helping RIGI at sensing pathogenic RNA (PubMed:30804210). May also act as a phosphorylation- dependent sequence-specific endoribonuclease in vitro: Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR (PubMed:11604510).
Cellular Location	Cytoplasm, cytosol. Perikaryon {ECO:0000250\|UniProtKB:P97855}. Cytoplasm, Stress granule. Nucleus Note=Cytoplasmic in proliferating cells (PubMed:11604510). Cytosolic and partially nuclear in resting cells (PubMed:11604510). Recruited to stress granules in response to arsenite treatment (PubMed:37672657, PubMed:20180778). The unphosphorylated form is recruited to stress granules (PubMed:37672657). HRAS signaling contributes to this process by regulating G3BP dephosphorylation (PubMed:37672657)
Tissue Location	Ubiquitous..

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR.

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¥ 1,250.00

Cat# AP75467

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[KD]G3BP Rabbit mAb

BACKGROUND

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